Abstract
Isolation of bacteria for antibacterial activity was conducted on sponge-associated marine bacteria, Haliclona sp.2 and Axinellid sp.from Ngge Island, Sibolga. Seventeen isolates were obtained from the two sponges. Based on antibacterial test, 11 isolates(64%)were potential as antibacterial, whereas 6 isolates (35%) were not indicatedas antibacterial. Isolate H2, A1, and A2 with broad spectra of antibacterial activity were further separated for extraction of secondary metabolites. Based on screening results of isolates, etil acetate extract of isolate H2,A1, A2 and n-hexana extract of isolate A2 displayed antibacterial against Staphylococcus aureusandEscherichia coli, whereas methanol extract A2 was only able to inhibit the growth of Escherichiacoli. For bioactive compound test, EH2, EA1 and EA2 were positive for alkaloid, whereas HA2 and MA2 were positive for alkaloid and saponin. Based on purification of secondary metabolite using Thin Layer Chromatography (TLC), there were22 fractions with solvents of different polarities. Screening of antibacterial activity for isolate extract fractions showed thatMA2 of fraction 2 had a strong inhibitive effectagainst E.coli and S. Aureusabout10,15 mm and 9,95 mm consecutively. The antagonistic activity of EH2of fraction 3 against S. aureusandE.coliwere about 7,05 mm and 7,45 mm consecutively, whereas EA1 of fraction 3 was about 6,55 mm againts P. Aeruginosa. The results of phytochemical secondary metabolite of isolate extract fractions showed that MA2 of fraction 2 and MA2 of fraction 3 werepositive for saponin, while EH2 fraction 3 and EA1 fraction 3 were positive for alkaloid. Bacteria associated with sponges Haliclona sp.2 and Axinellidsp. had activity as antibacterial against bacteria S. aureus, E. coli and P. aeruginosa.
Highlights
Isolate H2, A1, and A2 with broad spectra of antibacterial activity were further separated for extraction of secondary metabolites
Based on screening results of isolates, etil acetate extract of isolate H2,A1, A2 and n-hexana extract of isolate A2 displayed antibacterial against Staphylococcus aureusandEscherichia coli, whereas methanol extract A2 was only able to inhibit the growth of Escherichiacoli
It was put into oxoid disk that was droped with 10 μl of extract –n- hexana secondary metabolite potential bacteria into agar media that was inoculated with Staphylococcus Aureus, Pseudomonas aeruginosa andEscherichia coli
Summary
It was put into oxoid disk that was droped with 10 μl of extract –n- hexana secondary metabolite potential bacteria into agar media that was inoculated with Staphylococcus Aureus, Pseudomonas aeruginosa andEscherichia coli. It placed on the empty oxoid disk that already spotted with 10μl fraction n- hexana secondary metabolite of potential bacteria into media NA that was inoculated with bacteria S. aureus, P. aeruginosa and E. coli.The culture was incubated for 24 hours at 250 C and inhibition zone formed was observed withvernier caliper.
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