Abstract
The diversity, community structure and vertical distribution characteristics of bacteria in the surface and subsurface soil and water samples of a closed landfill in Shanghai Jiading District were investigated to reveal the relationships between natural waste degradation degree and the succession of bacterial community composition. High-throughput sequencing of bacteria 16S rDNA genes was used to analyze the bacterial community structure and diversity. The results showed that the diversity of bacteria in the surface samples was higher than that in the deep samples. Proteobacteria was the dominant phylum in all the samples, and the percentage increased with depth. At the genus level, Thiobacillus, Pseudomonas, Aquabacterium, and Hydrogenophaga were the dominant genera in surface, medium, deep and ultra-deep soils, respectively. The Bray–Curtis dissimilarity of the soil bacterial communities in the same layer was small, indicating that the community composition of the samples in the same layer was similar. The RDA result showed that ammonium, nitrate, pH and C/N significantly influenced the community structure of soil bacteria. This is of great relevance to understand the effect of natural waste degradation on bacterial communities in closed landfills.
Highlights
IntroductionPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations
The total nitrogen content was low in all soil samples, probably because the landfill was mainly formed by construction waste, in which the biologically
The diversity of microbial communities was mainly controlled by the soil layer, and the surface samples were more diverse while the deeper samples were relatively barren
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Landfill is one of the common methods for the treatment of municipal solid waste in China [1]. With rapid urbanization in China, cities are expanding in scale and suburban land is being developed. The landfills in suburban areas will be closed gradually [2,3]. Bacteria play an important role in promoting the natural degradation and removal of waste [4]. Microbial response to natural waste degradation in closed landfills around urban areas should be analyzed to ensure urban environmental safety
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
