Abstract
In this study, we used DGGE technique to analyze the existence of microbial community of compost. From ten samples extracted, isolated genomic and amplify 16SrDNA region by 27F and 907R primer. And then choice No.6 for analysis by DGGE method with primers (517F-GC, 357R and 517F, 357R) had result nine sequences of V3 region follow as: 6a1, 6a2, 6a3, 6a4, 6d1, 6d2, 6d3, 6e1 and 6e2. The analysis and compared with is database onto GeneBank for sequence similarities follow with Accession number: 6a1, 6a2 similarities 99% with JF987387.1 and JF828759.1, JF 989848.1 similarities 93% with 6a4 and 88% 6a3, 6d1 similarities 82% with AB206012.1, 6d2 similarities 95% JF429206.1, 6d3 similarities 86% with FN781411.1, 6e1 similarities with 99%JF176784.1 and FJ516783.1 similarities 87% with 6e2.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
