Bacteria can proliferate in thawed cryoprecipitate stored at room temperature for longer than 4h.

Abstract

Although key coagulation factor activities are maintained in thawed cryoprecipitate stored for up to 24h at ambient temperature, several jurisdictions limit such storage to 4-6h. Here, we separately spiked thawed cryoprecipitate units with four bacterial strains: Staphylococcus epidermidis, Serratia liquefaciens, Pseudomonas putida and Pseudomonas aeruginosa. No strains grew in the first 4h of storage, but by 24h, three of four exhibited up to 1000-fold proliferation. Pathogen inactivation technologies could be explored to mitigate the safety risk posed by extending storage of thawed cryoprecipitate at room temperature.