Abstract
A bacterial biosensor that immobilizes living bacteria of strain Bacillus subtilisAS 1.398 are coupled to a Clark-type oxygen electrode to determine hydrogen peroxide. The H 2O 2is catalyzed by catalase from the bacteria to produce H 2O and O 2. This sensor is advantageous in terms of linearity (10 to 2000 μ MH 2O 2), lifetime (65 days), and selectivity (no significant interference from some amino acids, metal ions, polyphenols, glucose, and ascorbic acid), especially because bacteria can be used as 2 catalase enzyme membrane that can reproduce itself. The correlation coefficient is 0.999.
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