Abstract
Rapid-scan electron paramagnetic resonance spectroscopy is an emerging technique which substantially improves the signal-to-noise ratio and time resolution compared to conventional continuous-wave experiments. This allows the investigation of spin-labeled biomolecules and their structural dynamics on much shorter time scales than usually accessible. The EPR spectrum however is superimposed by a strong background that is caused by microphonic effects of the alternating magnetic field. This article discusses the use of non-quadratic cost functions for background removal of rapid-scan spectra. The method is validated for the most prominent type of spin-probes in the field of biochemistry: the nitroxide spin-label.
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