Backcrossing of BC3F2 Accession with Local Parents of Rice Plants which Resistance to Submergence Stress

Abstract

The aims of this research was to know the introgression of Sub1 gene in BC4F1 which was backcrossed between BC3F2 with local parents. This reseach has been conducted in Green house of Agro Techno Park (ATC) and soaking tub at Sriwijaya University, Indralaya Ogan Ilir District. This research was conducted from September 2020 to March 2021. Rice seed of BC3F2 accessions of Pegagan and BC3F2 accessions of Pelita Rampak as male parents and two local genotypes of Pegagan and Pelita Rampak as female parents were used in this study. The methode being used during the research was backcrossing of local genotypes and accessions of BC3F2 to get accessions of BC4F1. Submergence tolerance was conducted during vegetative phase. The parameter being observed included percentage of crossing methods and number of rice seeds after backcrossing. While other parameters being obserbed included plant height, leaves Greenest, dry weight and growth analysis. The results showed that crossing between local genotyes and accession of BC3F2 showed that genotype of pelita rampak showed better growth after submergence indicated by better plant height, leaves greenest and growth analysis.