Back Cover: Studying the effect of microwave heating on the digestion process and identification of proteins

Abstract

ELECTROPHORESISVolume 38, Issue 3-4 Back CoverFree Access Back Cover: Studying the effect of microwave heating on the digestion process and identification of proteins First published: 22 February 2017 https://doi.org/10.1002/elps.201770026AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Graphical Abstract Shobha Devi, Bo-Hung Wu, Pei-Yu Chu, Yue-Pei Liu, Hsin-Lin Wu, and Yen-Peng Ho Electrophoresis 2017, 38, 429–440. DOI: 10.1002/elps.201600392 The Back Cover shows the impact of microwave irradiation on the in-solution digestion processes and the detection limit of proteins. Kinetic processes of many peptides produced through the trypsin digestion of various proteins under microwave heating at 50 °C revealed incomplete digestion. We examined the detection limits of individual proteins under microwave heating at 50 °C and at different time intervals (1, 5 and 30 min) using LC-MS. We conclude that, if the peptides without missed cleavage dictate the detection limit, conventional digestion will lead to a better detection limit. Volume38, Issue3-4February 2017 RelatedInformation