Back Cover: Optimized two‐dimensional gel electrophoresis in an alkaline pH range improves the identification of intracellular CFDA‐cisplatin‐protein adducts in ovarian cancer cells

Abstract

DOI: 10.1002/elps.201700377 The cover picture shows the visualization of CFDA-cisplatin-protein adducts through 2DE. Human ovarian carcinoma cell lines A2780 and A2780cis cells were treated with CFDA-cisplatin and separated by 2DE. The use of fluorescent CFDA-cisplatin enables the visualization of CFDA-cisplatin-protein adducts. For the image analysis the fluorescence scans from CFDA-cisplatin-protein adducts and the Coomassie staining were fused into one image. The identification of nine previously unknown intracellular binding partners of CFDA-cisplatin by mass spectrometry provides new insights into the cellular processing of cisplatin.