Abstract
Bacillus velezensis 83 was isolated from mango tree phyllosphere of orchards located in El Rosario, Sinaloa, México. The assessment of this strain as BCA (biological control agent), as well as PGPB (plant growth-promoting bacteria), were demonstrated through in vivo and in vitro assays. In vivo assays showed that B. velezensis 83 was able to control anthracnose (Kent mangoes) as efficiently as chemical treatment with Captan 50 PH™ or Cupravit hidro™. The inoculation of B. velezensis 83 to the roots of maize seedlings yielded an increase of 12% in height and 45% of root biomass, as compared with uninoculated seedlings. In vitro co-culture assays showed that B. velezensis 83 promoted Arabidopsis thaliana growth (root and shoot biomass) while, under the same experimental conditions, B. velezensis FZB42 (reference strain) had a suppressive effect on plant growth. In order to characterize the isolated strain, the complete genome sequence of B. velezensis 83 is reported. Its circular genome consists of 3,997,902 bp coding to 3949 predicted genes. The assembly and annotation of this genome revealed gene clusters related with plant-bacteria interaction and sporulation, as well as ten secondary metabolites biosynthetic gene clusters implicated in the biological control of phytopathogens. Despite the high genomic identity (> 98%) between B. velezensis 83 and B. velezensis FZB42, they are phenotypically different. Indeed, in vitro production of compounds such as surfactin and bacillomycin D (biocontrol activity) and γ-PGA (biofilm component) is significantly different between both strains.
Highlights
Bacillus is a bacterial genus which comprises several species that establish, directly or indirectly, beneficial relations with plants (Olanrewaju et al 2017)
B. velezensis 83 is a biological control agent and plant growth‐promoting bacteria Mango anthracnose severity was reduced in trees treated with B. velezensis 83 at three different doses, and fruit damage was to those fruits harvested from trees with chemical conventional treatments (Captan 50 PHTM or Cupravit hydroTM)
When chemical products are used for the control of anthracnose, it is important to ensure that the maximum residue limits (MRLs) of pesticides are under the maximal concentration allowed by international standard
Summary
Bacillus is a bacterial genus which comprises several species that establish, directly or indirectly, beneficial relations with plants (Olanrewaju et al 2017). The strains B. velezensis UCMB5044 (a oligotrophic PGPR isolated from desert soils), B. velezensis UCMB5113 and B. velezensis At1 (both strain plant endophytes) and B. velezensis UCMB55007 (a copiotroph isolated from calf gut) share 99% of genetic homology. Their gene expression in response to root maize exudates were significantly different because they possess different DNA methylation patterns (Reva et al 2019)
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