Bacillus urbisdiaboli sp. nov., isolated from soil sampled in Xinjiang

Abstract

A rod-shaped, endospore-forming, facultative anaerobic bacterium, designated FJAT-45385T, was isolated from soil collected from Devil City in the Xinjiang Autonomous Region in China. Growth was observed at 20-40 °C (optimum, 30 °C), pH 7.0-11.0 (pH 9.0) and in 0-10.0 % NaCl (4 %), respectively. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7. The main fatty acids were anteiso-C15 : 0 (37.4 %), iso-C15 : 0 (15.1 %) and C16 : 0 (12.6 %). The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-45385T to the genus Bacillus, and showed the highest sequence similarity to Bacillus wakoensis DSM 2521T (96.0 %). The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-45385T and its closest related species were 67.8 and 35.5 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belong to a different taxon. The DNA G+C content was 38.1 mol%. The phenotypic characters and taxono-genomics study revealed that strain FJAT-45385T represents a novel Bacillus species, for which the name Bacillus urbisdiaboli sp. nov. is proposed. The type strain is FJAT-45385T (=DSM 104651T=CCTCC AB 2016263T).