Abstract
Transcription of the hbs gene under vegetative growth condition is subject to repression when cells enter in late exponential phase. We have determined the sites at which transcription of the hbs gene initiates in vitro. On a supercoiled template, transcription of the hbs gene is initiated by sigmaARNAP at two overlapping hbs promoters (P1 and P3). We have demonstrated that highly purified Hbsu protein acts as a repressor of its own synthesis. The binding of the sequence-independent DNA-binding and DNA-bending Hbsu protein does not seem to exclude sigmaARNAP from the promoters. In this report we show that Hbsu, in vitro, does not repress transcription by a mere steric hindrance on sigmaARNAP binding.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
