Abstract
Background Colletotrichum and Fusarium species are among pathogenic fungi widely affecting Coffea arabica L., resulting in major yield loss. In the present study, we aimed to isolate bacteria from root rhizosphere of the same plant that is capable of antagonizing Colletotrichum gloeosporioides and Fusarium oxysporum as well as promotes plant growth.ResultsA total of 42 Bacillus species were isolated, one of the isolates named BT42 showed maximum radial mycelial growth inhibition against Colletotrichum gloeosporioides (78%) and Fusarium oxysporum (86%). BT42 increased germination of Coffee arabica L. seeds by 38.89%, decreased disease incidence due to infection of Colletotrichum gloeosporioides to 2.77% and due to infection of Fusarium oxysporum to 0 (p < 0.001). The isolate BT42 showed multiple growth-promoting traits. The isolate showed maximum similarity with Bacillus amyloliquefaciens.Conclusion Bacillus species (BT42), isolated in the present work was found to be capable of antagonizing the pathogenic effects of Colletotrichum gloeosporioides and Fusarium oxysporum. The mechanism of action of inhibition of the pathogenic fungi found to be synergistic effects of secondary metabolites, lytic enzymes, and siderophores. The major inhibitory secondary metabolite identified as harmine (β-carboline alkaloids).
Highlights
Colletotrichum and Fusarium species are among pathogenic fungi widely affecting Coffea arabica L., resulting in major yield loss
The antagonistic effect of Bacillus species isolates against C. gloeosporioides and F. oxysporum Bacteria isolates were studied against two fungal pathogens C. gloeosporioides and F. oxysporum for radial mycelial growth inhibition
Identification of isolate The isolate BT42 selected for identification because it showed highest mycelial growth inhibition of C. gloeosporioides and F. oxysporum when compared to other isolates
Summary
Colletotrichum and Fusarium species are among pathogenic fungi widely affecting Coffea arabica L., resulting in major yield loss. The word coffee comes from the name of the place in Ethiopia called “Kaffa”. There are many species of coffee, but the two most widely cultivated are C. arabica L. and C. canephora (robusta). Of the total world production of coffee, C. arabica L. takes the lion’s share, which is 66% and C. canephora only of 34%. Coffee produced in few countries, it is the most traded agricultural products around the globe after oil. In Ethiopia, it is mostly exported cash crop that accounts for 69% of all agriculturally export commodities and it was estimated that at least 15 million of Ethiopian population depend directly or indirectly on coffee production
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
