Bacillus sp. SH-517에 의한 keratinase의 생성 최적 배양 조건

Abstract

A strain SH-517 which produce extracellular keratinase, was isolated from the soil of a poultry waste and a poultry factory. An isolate SH-517 was identified as Bacillus sp. based on its morphological and biochemical characteristics. The optimal culture conditions for the production of keratinase by Bacillus sp. SH-517 were investigated. The optimal medium composition for keratinase production was determined to be 2.0% chicken feather as carbon source, 0.5% beef extract as organic nitrogen source, 0.5% as inorganic nitrogen source and 0.06% KCl, 0.05% NaCl, 0.04% , 0.03% as mineral source and 0.01% yeast extract as growth factor. The optimal temperature and pH of medium were shown and 8.5 with shaking culture (180 rpm/min), respectively. The maximum keratinase production reached maximum of 125 units/ml/min after 42 hr of cultivation under the optimal culturing conditions.