Bacillus Pumilus as a Supplement for Waste Recycling by Insect

Abstract

The possibility of enriching the intestinal microbiota of cockroaches of the species Pycnoscelus nigra with a cellulolytic strain - Bacillus pumilus IB-320 was reviewed, as well as an assessment of the effect of the used microorganism on process of bioconversion of various types of paper waste and soil humification. Based on the results of a model experiment with a period of 90 days, various degree of bioconversion of cellulose-containing wastes for the zoomicrobial comple were found (in descending order): grass hay, paper and soil substrate (mix of brick crumbs, softwood sawdust and coconut-fiber substrate). Treatment with strain IB-320 contributed to an increase in the total number of bacteria obtained from the substrates and fecal extracts of cockroaches by 1.5-1.7 and 1.5-3 times, respectively. The introduction of the selected strain also significantly increased the rate of destruction of such substrates as cardboard and egg trays by 40% and 25%, accordingly. In the variant with using the grass hay, the presence of strain IB-320 led to an increase in humic acids by 1.5. The prospects of application Bacillus pumilus IB-320 as part of an active zoomicrobial complex for more efficient disposal of cellulose-containing waste have been shown.