Abstract
Five different Brazilian soils were examined for the incidence of Bacillus nitrogen fixers. These counts were set in relation to the total spore count, and the number of facultative anaerobic spores. Twenty-four Bacillus strains were isolated using media lacking a nitrogen source, identified and their acetylene-reducing ability was determined. Eighteen of these strains were identified asB. polymyxa, and the other 6 were related to this species, but were different in some taxonomical tests. Of the 24 isolated strains, only 13 reduced acetylene; similarly, only 3 out of 9B. polymyxa reference strains were able to reduce acetylene.
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