Abstract
RNA-seq analysis of B. megaterium exposed to pH 7.0 and pH 4.5 showed differential expression of 207 genes related to several processes. Among the 207 genes, 11 genes displayed increased transcription exclusively in pH 4.5. Exposure to pH 4.5 induced the expression of genes related to maintenance of cell integrity, pH homeostasis, alternative energy generation and modification of metabolic processes. Metabolic processes like pentose phosphate pathway, fatty acid biosynthesis, cysteine and methionine metabolism and synthesis of arginine and proline were remodeled during acid stress. Genes associated with oxidative stress and osmotic stress were up-regulated at pH 4.5 indicating a link between acid stress and other stresses. Acid stress also induced expression of genes that encoded general stress-responsive proteins as well as several hypothetical proteins. Our study indicates that a network of genes aid B. megaterium G18 to adapt and survive in acid stress condition.
Highlights
Bacteria have innate ability to survive and grow in several stress conditions including extreme ecological niches
We previously reported the predominance of the genus Bacillus in acidic soil condition of Assam based on culture-dependent assay and isolation of an isolate of B. megaterium G18 that could grow at pH 4.514
Low pH in the environment is known to influence bacterial growth characteristics; they are able to adapt to environmental stress conditions including acid stress through deployment of several mechanisms
Summary
Bacteria have innate ability to survive and grow in several stress conditions including extreme ecological niches. We previously reported the predominance of the genus Bacillus in acidic soil condition of Assam based on culture-dependent assay and isolation of an isolate of B. megaterium G18 that could grow at pH 4.514. This isolate was used in the present study to decipher the underlying mechanism of acid tolerance through a differential transcriptome profiling. We report the transcriptome profile of B. megaterium G18 subjected to acid stress condition (pH 4.5) and neutral condition (pH 7.0) using the generation sequencing technology. To the best of our knowledge, this is the first differential transcriptomics analysis of the response of B. megaterium G18 to an acidic stress condition
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