Abstract
In this article, the supernatant of the soil-borne pathogen Bacillus mn14 was used as the catalyst for the synthesis of AgNPs. The antibacterial and antifungal activity of Bs-AgNPs was evaluated, in which S. viridans and R. solani showed susceptibility at 70 µL and 100 µL concentrations. Enzyme properties of the isolates, according to minimal inhibitory action and a growth-enhancing hormone–indole acetic acid (IAA) study of the isolates, were expressed in TLC as a purple color with an Rf value of 0.7. UV/Vis spectroscopy revealed the presence of small-sized AgNPs, with a surface plasmon resonance (SPR) peak at 450 nm. The particle size analyzer identified the average diameter of the particles as 40.2 nm. The X-ray diffraction study confirmed the crystalline nature and face-centered cubic type of the silver nanoparticle. Scanning electron microscopy characterized the globular, small, round shape of the silver nanoparticle. AFM revealed the two-dimensional topology of the silver nanoparticle with a characteristic size ranging around 50 nm. Confocal microscopy showed the cell-wall disruption of S. viridans treated with Bs-AgNPs. High-content screening and compound microscopy revealed the destruction of mycelia of R. solani after exposure to Bs-AgNPs. Furthermore, the Bs-AgNPs cured sheath blight disease by reducing lesion length and enhancing root and shoot length in Oryza sativa seeds. This soil-borne pathogen Bacillus-mediated synthesis approach of AgNPs appears to be cost-efficient, ecofriendly, and farmer-friendly, representing an easy way of providing valuable nutritious edibles in the future.
Highlights
Introduction distributed under the terms andThe distinctive physicochemical properties of AgNPs have attracted the attention of the technical community [1], e.g., their elevated thermal conductivity, chemical permanence, and antibacterial effects [2]
Among the 27 isolates Bacillus mn14, mn5, and mn15 exhibited positive results for indole acetic acid (IAA), whereas no color change was observed for siderophore and phosphate solubilization
A light-purple spot was later identified by spraying with ninhydrin, and the
Summary
The distinctive physicochemical properties of AgNPs have attracted the attention of the technical community [1], e.g., their elevated thermal conductivity, chemical permanence, and antibacterial effects [2]. Nanotechnology and nanoscale materials have emerged as potent delivery methods for several ailments. Microbial-mediated synthesized nanoparticles toward microbial multidrug resistance and microbial biofilm creation [3] are considered an alternative to germicidal agents [4]. The antimicrobial activities of microbial-mediated synthesized AgNPs are impressive [5]. The synthesis of microbial-mediated synthesized AgNPs aligns well with green chemistry values, and the viridescent fusion of AgNPs results in an environmentally friendly material which is nonhazardous to all living organisms [6]. The natural and artificial (chemical) products facilitate living status.
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