Abstract
Two bacterial strains (JC247T and JC248) were isolated from soil samples collected from Rann of Kutch, Gujarat, India. Colonies of both strains were creamy white. Cells were Gram-stain-positive, rods-to-curved rods (crescent-shaped), and produced centrally located oval-shaped endospores. Major (>5 %) fatty acids of both strains were iso-C16 : 0, iso-C14 : 0, iso-C15 : 0, C16 : 1ω11c and C16 : 0, with minor ( < 5 but >1 %) amounts of anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 1 H, iso-C17 : 0, iso-C18 : 0, C14 : 0, C17 : 0, C18 : 0, C18 : 1ω9c, iso-C17 : 1ω10c and anteiso-C17 : 0B/isoI. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids of both strains. Cell-wall amino acids were l-alanine, d-alanine, d-glutamic acid and meso-diaminopimelic acid. The genomic DNA G+C content of strains JC247T and JC248 was 48.2 and 48.1 mol%, respectively. Both strains were closely related with mean DNA-DNA hybridization >90 %. 16S rRNA gene sequence analysis of both strains indicated that they are members of the genus Bacillus within the family Bacillaceae of the phylum Firmicutes. Both strains had a 16S rRNA gene sequence similarity of 96.93 % with Bacillus firmus NCIMB 9366T and < 96.92 % with other members of the genus Bacillus. Sequence similarity between strain JC247T and JC248 was 100 %. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strains JC247T and JC248 as representatives of a novel species of the genus Bacillus, for which the name Bacilluscrescens sp. nov. is proposed. The type strain is JC247T ( = KCTC 33627T = LMG 28608T).
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