Abstract
This study was carried out in order to investigate the presences of Bacillus cereus and its enterotoxigenic genes in infant foods in Isfahan, Iran. Overall 200 infant foods with various based were collected and immediately transferred to the laboratory. All samples were culture and the genomic DNA was extracted from colonies with typical characters of Bacillus cereus. The presences of enterotoxigenic genes were investigated using the PCR technique. Eighty-four of two hundred samples (42%) were found to be contaminated with B. cereus with a ranges of 3 × 101–9.3 × 101 spore per gram sample. Totally, entFM had the highest (61.90%) incidences of enterotoxigenic genes while hblA had the lowest (13.09%) incidences of enterotoxigenic genes. Overall, 6.7% of B. cereus isolates had all studied enetrotoxigenic genes while 25.5% of B. cereus strains had all studied enetrotoxigenic genes expectance bceT gene. Thisstudyisthe first prevalence report of B. cereus and its enterotoxigenic genes in infant foods in Iran. Results showed that the infant food is one of the main sources of enterotoxigenic genes of B. cereus in Iran. Therefore, the accurate food inspection causes to reducing outbreak of diseases.
Highlights
Baby foods are the primary source of nutrition for kids before they are able to digest other types of food
The suspension was streaked onto chromogenic B. cereus agar (BCA) supplemented with chromogenic B. cereus selective supplement (Oxoid)
The presence of B. cereus in typical colonies has been confirmed by the polymerase chain reaction (PCR) techniques
Summary
Baby foods are the primary source of nutrition for kids before they are able to digest other types of food. Statistical analysis showed significant differences (P < 0.05) for the presence and spore ranges of B. cereus between baby food with rice and milk based and baby food with wheat, banana, and milk based which was in agreement with the results of Reyes et al [20]. Reyes et al [20] showed that 35 out of 56 baby foods with rice and milk based (62.5%) were contaminated with B. cereus with a range of 3 to over than 1000 spores per gram sample.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
