Abstract
Bacillus cereus TKU028, a chitinase and protease-producing strain, was isolated from the soil in Taiwan, by using shrimp head powder (SHP) as the sole carbon/nitrogen source. A chitinase (CHT) and a protease (PRO) were purified by chitin affinity binding and casein affinity binding, respectively. The molecular masses of CHT and PRO determined by SDS- PAGE were approximately 40 kDa and 43 kDa, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of CHT and PRO were pH 5-6, 60 ℃, pH 5-8, and ≦60 ℃, and pH 9,50-60 ℃, pH 5-9, and ≦ 40℃, respectively. CHT was little inhibited by Mn2+ and EDTA, and PRO was inhibited by Cu2+ , EDTA, and 2 mM SDS. The chitinase-hydrolyzed products of subtract degradation were analyzed by HPLC at 1-48 h intervals. It was observed the major products are (GlcNAc)6. In addition, B. cereus TKU028 has unlimited potential to enhance the biodegradtion of shrimp shells in the seawater containing mangrove river sediment.
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