Bacillus cereus enhances salt tolerance of cucumber seedlings by improving antioxidant metabolism and decreasing the ion toxicity

Abstract

Salt stress poses a significant challenge to plants, exerting a detrimental impact on crop growth and yield. This study investigated the effects of the strain Bacillus cereus on plant growth, ion contents and antioxidant metabolism of cucumber seedlings under salt stress (150 mM NaCl). The results showed that B. cereus could colonize the roots system of cucumber, with peak colonization occurring on the 3rd day. Inoculation with B. cereus effectively alleviated the growth inhibition of cucumber seedlings induced by salt stress, resulting in improvements in plant height, stem diameter, fresh and dry weight. Furthermore, the Na+ content in cucumber seedlings subjected to salt stress was significantly reduced by 38.35 % in leaves and 38.19 % in roots upon B. cereus inoculation, while K+ content increased by 9.88 % and 168.34 % in leaves and roots, respectively. Additionally, B. cereus significantly enhanced antioxidant enzyme activity and antioxidant content, while reduced the levels of H2O2, MDA, and O2−. in cucumber seedlings under salt stress conditions. B. cereus-treated seedlings exhibited an increase in soluble sugar content by 35.27 % and 93.21 % in leaves, and 59.36 % and 128.78 % in roots, respectively. Moreover, B. cereus treatment significantly up-regulated the expression of salt tolerant gene in both cucumber leaves and roots. These results showed that B. cereus enhanced salt tolerance in cucumber seedlings by modulating the antioxidant defense system, maintaining ion homeostasis, and promoting the expression of stress-related genes, ultimately improving the growth of cucumber seedlings.