Abstract
BackgroundThe Bacillus anthracis S-layer protein, BslA, plays a crucial role in mammalian infection. BslA is required to mediate adherence between host cells and vegetative forms of bacteria and this interaction promotes target organs adherence and blood–brain barrier (BBB) penetration in vivo. This study attempts to identify the potential eukaryotic ligand(s) for B. anthracis BslA protein.ResultsBiochemical approaches have indicated that the putative host cell ligand(s) for BslA is a surface protein, which is independent of the sugar components for binding to Bs1A. A ligand screening using blot overlays, far Western blots and mass spectrometry analyses revealed that BslA binds to mammalian laminin. ELISA based solid-phase binding assays and surface plasmon resonance assays demonstrated that there were high affinity interactions between BslA(260–652) and laminin. The SPR results also revealed the dissociation constants values of 3.172 × 10−9M for the binding of BslA(260–652) to laminin.ConclusionsThese data demonstrated that laminin is a ligand for BslA.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0802-8) contains supplementary material, which is available to authorized users.
Highlights
The Bacillus anthracis S-layer protein, B. anthracis S-layer protein A (BslA), plays a crucial role in mammalian infection
Bacterial adhesins bind to host cell ligands via a very specific process, and the specificity of this match determines the range of host cells susceptible to infection by a particular strain of pathogen [3]
The amino acid sequence analysis of BslA(260–652) using NPS@ supported the results of the Circular dichroism (CD) spectrum analysis (Table 1)
Summary
The Bacillus anthracis S-layer protein, BslA, plays a crucial role in mammalian infection. The key to bacterial infections of host tissues is the establishment of a stable association between the bacterium and host surface structures. This process is essential for many bacteria for withstanding cellular mechanical cleansing processes and to allow invasion [1]. Most microbes have specific adhesins, which can bind to specific ligands on the surface of the host cell [2]. Bacterial adhesins bind to host cell ligands via a very specific process, and the specificity of this match determines the range of host cells susceptible to infection by a particular strain of pathogen [3].
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