Abstract
Tomato (Solanum lycopersicum), as an important economical vegetable, is often infected with Rhizoctonia solani, which results in a substantial reduction in production. Therefore, the molecular mechanism of biocontrol microorganisms assisting tomato to resist pathogens is worth exploring. Here, we use Bacillus amyloliquefaciens SN16-1 as biocontrol bacteria, and employed RNA-Seq technology to study tomato gene and defense-signaling pathways expression. Gene Ontology (GO) analyses showed that an oxidation-reduction process, peptidase regulator activity, and oxidoreductase activity were predominant. Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that phenylpropanoid biosynthesis, biosynthesis of unsaturated fatty acids, aldosterone synthesis and secretion, and phototransduction were significantly enriched. SN16-1 activated defenses in the tomato via systemic-acquired resistance (which depends on the salicylic acid signaling pathway), rather than classic induction of systemic resistance. The genes induced by SN16-1 included transcription factors, plant hormones (ethylene, auxin, abscisic acid, and gibberellin), receptor-like kinases, heat shock proteins, and defense proteins. SN16-1 rarely activated pathogenesis-related proteins, but most pathogenesis-related proteins were induced in the presence of the pathogens. In addition, the molecular mechanisms of the response of tomatoes to SN16-1 and R. solani RS520 were significantly different.
Highlights
Tomato (Solanum lycopersicum) is an economically important vegetable throughout the world, with significant importance for human health and nutrition
B. amyloliquefaciens has been developed as biological control agent to control plant pathogens
RNA-Seq was employed to investigate the changes in gene expression in tomato plants after inoculation with B. amyloliquefaciens SN16-1 and RS520
Summary
Tomato (Solanum lycopersicum) is an economically important vegetable throughout the world, with significant importance for human health and nutrition. Mainly caused by Rhizoctonia solani, is a common disease that results in a substantial reduction in production [1]. Chemical methods are used to control the disease, but such pesticides leave residues and may produce adverse effects on the environment and human health [2]. Effective means that accord with the sustainable development of agriculture are being sought to manage this disease. Biological control is a promising alternative to the use of chemicals to protect the plants from the pathogen [3,4]. Bacillus amyloliquefaciens is a biological control agent that can control damping off [5,6]
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