Abstract

Abstract Bacillus amyloliquefaciens M27 was selected as a control agent for the biological control ofcucumber powdery mildew. The new mixture of B.amyloliquefaciens M27 and plant (Eucalytus) extract wasdeveloped to improve the control activity of B.amyloliquefaciens M27 against cucumber powdery mildew. Themixed formulation showed the high preventive and curative control effect against cucumber powdery mildewwhen it was diluted at 500 times and foliar-sprayed. Its control effect was higher in preventive spraying thancurative spraying. When 500-fold diluted solution of the formulation was sprayed preventively four times atfive-day intervals, three times at seven-day intervals and twice at ten-day intervals, the diseased leaf area wasshown to be 4.4%, 8.0%, 27.9%, respectively; Whereas the diseased leaf area in the control plot was 45.4%.When the 500-fold diluted formulation was sprayed curatively four times at five-day intervals, three times atseven-day intervals and twice at ten-day intervals after occurred cucumber powdery mildew, the diseases leafarea was 11.5%, 25.2%, 51.8%, respectively; whereas in the control plot, the diseases leaf area was 64.3%.When the 500-fold diluted formulation was treated four times at five-day intervals in the plastic house, itscontrol effect was higher than that treated three times at seven-day intervals and twice at ten-day intervals. Asthe results, the mixed formulation of B.amyloliquefaciens M27 and plant extract could be a promisingcandidate of bio-fungicides for the environment-friendly control of powdery mildew of cucumber.Key words Bacillus amyloliquefaciens M27, Cucumber Powdery mildew, Plant Extract

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