Bacillus amyloliquefaciens-9 Reduces Somatic Cell Count and Modifies Fecal Microbiota in Lactating Goats.

Yongtao Li,Nannan Jiang,Jianxin Liu,Hengbo Shi,Zhengbing Lv,Wenying Zhang

doi:10.3390/md19080404

Yongtao Li, Nannan Jiang + Show 4 more

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https://doi.org/10.3390/md19080404

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Journal: Marine Drugs	Publication Date: Jul 21, 2021
Citations: 5	License type: CC BY 4.0

Affiliation: Zhejiang University, Zhejiang Sci-Tech University

Abstract

Subclinical mastitis is one of the major problems affecting dairy animals’ productivity and is classified based on milk somatic cell counts (SCC). Previous data showed that marine-derived Bacillus amyloliquefaciens-9 (GB-9) improved the immunity and the nonspecific immune defense system of the body. In this study, the potential role of GB-9 in improving subclinical mastitis was assessed with Radix Tetrastigmae (RT) as a positive control in subclinical mastitis Saanen dairy goats. The current data showed that GB-9 and RT significantly reduced the SCC in dairy goats. After being fed with GB-9 or RT, the decreased concentrations of malondialdehyde, IgA, IgM, IL-2, IL-4, and IL-6 were observed. The amplicon sequencing analysis of fecal samples revealed that GB-9 significantly altered the bacterial community. Bacteroides and Phascolarctobacterium were the major genera that respond to GB-9 feeding. The correlation analysis using weighted gene co-expression network analysis showed a MePink module was most associated with the serum concentrations of immunoglobulin and interleukin. The MePink module contained 89 OTUs. The feeding of GB-9 in decreasing the SCC was associated with the altered abundance of Bacteroides, which was correlated with the concentrations of immunoglobulins and chemokines. Collectively, the current data suggested that marine-derived GB-9 could be a helpful probiotic to control subclinical mastitis.

Highlights

IntroductionPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations
The data of milk yield, dry matter intake, milk composition were compared among the group fed with basal diet (CS), the group fed with a basal diet supplemented with 0.3% GB-9 (w/w) (BS) [12], and the group fed with basal diet supplemented with 0.3%
All goats were from the same farm and were divided into three groups as follows: goats fed with basal diet (CS, 6 goats), goats were fed the basal diet supplemented with 0.3% GB-9 (w/w) (BS, 10 goats) [12]; and goats fed with a basal diet supplemented with 0.3% Radix Tetrastigmae (RT) (w/w) (RS, 7 goats)

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Mastitis is a major problem in dairy herds worldwide and causes considerable economic losses [1,2]. Intramammary infections with various pathogenic bacteria often cause mastitis. The mammary gland attracts immune cells, such as white blood cells, to clear the bacteria, resulting in an elevation in the milk somatic cell count (SCC). Subclinical mastitis accounts for the majority of mastitis cases and is characterized by an increase in SCC (value > 200 × 104 /mL for goat milk) without clinical signs in the appearance of milk or the udder, as well as decreased milk production and altered milk composition [3]

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