Abstract
Bio-renovation is the microorganism-assisted conversion of the structure and improvement of biological efficacies of natural products and synthetic compounds. In this study, we investigated the effect of plant callus on anti-melanogenic effect and compared the activity changes of bio-renovated callus. Phryma leptostachya bio-renovation products at the concentrations of 100, 200, 400 μg/mL suppressed tyrosinase activity and the expression of melanogensis-related protein such as tyrosinase-related protein-1 (TRP-1), tyrosinase-related protein-2 (TRP-2) and microphthalmia-associated transcription factor (MITF) without a loss of cell viability in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16F10 melanoma cells relative to its substrate. Moreover, cell viabilities of PLBR increased over the tested concentrations compared to its substrate (PL). PLBR at 400 μg/mL inhibited the α-MSH-induced melanogensis effect to the level of non-treated control. These results indicate that the structure of various compounds contained in Phryma leptostachya (PL) were modified through a bio-renovation technique and which conferred anti-melanogenic properties to PL.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
