B16/F10 흑색종양세포에서 삼내자 메탄올 추출물의 멜라닌 생성에 미치는 억제효과

Abstract

Abstract Objective: Recently the demands for the effective and safe depigmentative and anti-aging agents of the skin have increased due to the medical, pharmaceutical and cosmetic reasons. The purpose of this study is to investigate the MKG(Methanol Extract of Kaempferia galanga) and their dermal bioactivity properties related to cosmeceuticals such as depigmentation. Methods: We assessed inhibitory effects of MKG on melanin production in B16/F10 melanoma cells, on mushroom tyrosinase activity, effects of MKG on the expression tyrosinase, TRP-1, TRP-2, GSK-3β, CREB, MITF in B16/F10 melanoma cells without cytotoxicity range. Cell viab ility was measured by MTT assay and tyrosinase activity was assessed using by DOPA staining, western-blot anal ysis. We measured inhibition of melanin synthesis and tyrosinase act ivity by down-regulation of melanogenic enzyme expressions in α-MSH induced melanogenesis B16/F10 melanoma cel ls. Results: MKG inhibited tyrosinase-activity, total melanin contents and d endrite out-growth. MKG inhibited melanogenesis by down-regulation of tyorsinase, TRP-1, TRP-2, C REB, and MITF in B16/F10 cells. The treatment with MKG at the 12.5, 25 ㎍/㎖ level significantly inhibited the melanin synthesis induced α-MSH in B16/F10 melanoma cells compared with untreated control.