B12 production by marine microbial communities and Dinoroseobacter shibae continuous cultures under different growth and respiration rates

Abstract

AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials AME 83:251-262 (2019) - DOI: https://doi.org/10.3354/ame01921 B12 production by marine microbial communities and Dinoroseobacter shibae continuous cultures under different growth and respiration rates Josué Villegas-Mendoza1,*, Ramón Cajal-Medrano2, Helmut Maske2 1UABC, Facultad de Ciencias Marinas, Carretera Tijuana-Ensenada km 106, Ensenada, Baja California CP 22860, Mexico 2CICESE, Carretera Tijuana-Ensenada No. 3918, Ensenada, Baja California CP 22860, Mexico *Corresponding author: jvillegas18@uabc.edu.mx ABSTRACT: In situ dissolved B12 concentration in marine ecosystems is controlled by the balance between rates of release of B12 by prokaryotes, uptake by prokaryotes and eukaryotes, and abiotic degradation. We used chemostats at a range of specific growth rates (μ, d-1; 0.1 to 1) with natural communities of prokaryotes and monospecific cultures of a B12 producer, Dinoroseobacter shibae. We measured the dissolved B12 concentration produced in the culture (B12-d), the B12 in the particulate fraction (B12-p), cell concentration, respiration rate, particulate organic carbon and nitrogen (POC, PON), and the 16S amplicon composition. Total dissolved B12 concentrations (0.92 to 4.90 pmol l-1) were comparable to those found in the surface ocean. B12-p concentration was 6 to 35 times higher than B12-d. B12-d, B12-p, and community composition showed no relation to μ for either natural populations or D. shibae. The chemostats allowed calculation of the rates of production: B12-d (0.34 ± 0.28 pmol l-1 d-1) and B12-p (5.65 ± 2.34 pmol l-1 d-1), and the B12 cell quota (900 to 3300 molecules cell-1). In multispecies and D. shibae cultures, B12 production rates per cell increased with respiration rates (volumetric or per cell), and with rates of cellular organic carbon and nitrogen production. Rates increased with μ, but not the concentrations of B12-d or of B12-p. To understand the physiological and ecological dynamics of B12, concentrations alone are insufficient since they do not provide rates, which are important in understanding the dynamics between producers and consumers. KEY WORDS: Marine bacteria · Intracellular and dissolved B12 · Continuous cultures Full text in pdf format Supplement 1Supplement 2 PreviousNextCite this article as: Villegas-Mendoza J, Cajal-Medrano R, Maske H (2019) B12 production by marine microbial communities and Dinoroseobacter shibae continuous cultures under different growth and respiration rates. Aquat Microb Ecol 83:251-262. https://doi.org/10.3354/ame01921 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AME Vol. 83, No. 3. Online publication date: October 30, 2019 Print ISSN: 0948-3055; Online ISSN: 1616-1564 Copyright © 2019 Inter-Research.