B015 Differential modulation of oxidative and nitrosative stress pathways by red wine polyphenols, provinols™, in tissues from zucker fatty rats (FA/FA)

Abstract

Obesity is associated with numerous complications including significantly increased risks of diabetes and cardiovascular diseases. Epidemiological studies report an inverse association between dietary flavonoid consumption and mortality from cardiovascular diseases. The aim of this work was to study the effects of dietary supplementation of red wine polyphenols extract, Provinols™, on the regulation of both NO and O2− pathways in different tissues in an experimental model of obesity, the Zucker fatty rats (ZF). Rats received normal diet (n = 6) or supplemented with Provinols™ (20 mg/kg/day, n = 6) for 8 weeks. Then, NO and superoxide anion (O2−) measurement was carried out in heart, lung, and liver by electronic paramagnetic resonance after animals being scarified. Also, tissues were dissected and homogenized for western blot assays protein expression. Table 1: Western blots analysis of protein expression in tissues from ProvinolsTM−treated rats. Results are expressed as increase (+++) or decrease (−−−) of protein expression in tissues from Provinols TM-treated rats compared to controls. ns, no significant change. Regarding NO, we found that ProvinolsTM increased its release in both heart and lung, but not in liver. However, ProvinolsTM reduced O2− production in lung and liver without change in heart. In conclusion, Provinols™ differentially affect the balance between NO and O2−, as well as the associated regulation of protein expression, in tissues from obese rats.