フルオロメーターによる乳製品のビタミンB<sub>2</sub>定量について

Abstract

Fluorometric determination of riboflavin in milk products by electronic photofluorometer was studied. The conditions for photolysis.of riboflavin in milk products were examined(Table 1_??_7), and both from quantities of riboflavin added to deproteinized solution of milk products and from readings measured after photolysis, regression line was obtained by the application of methods of least squares (Table 8). Obtained regression coefficients in different kinds of milk products were shown in Table 9. Mean regression coefficient and estimated error with 5% of significant level obtained from measured samples of 188 were found to be 171.800 and 3.59. So the relation between reading in photofluorometer _??_ and content of riboflavin Y was shown by the next equation. _??_=Y/171.800=0.582072Y From the results of experiments, our method of determination of riboflavin in milk products by electronic photofluorometer is presented in this paper, and it was found to be well available for its determination. Contents of riboflavin in some Japanese milk products were also determined (Table 10).