Abstract
1). The vitamin B12 content in pyloric coeca of skipjack was determined by Euglena method and by Lactobacillus leichmanannii method. The average content of B12 determined by the former method was 30γ% on dry matter (Table 1), while 40γ% by the latter method (Table 2). In both methods, the values estimated with hot water extracts were lower than those determined with extracts obtained by enzymatic digestion (Tables 1 and 2). 2). Seasonal variation of B12 content in pyloric coeca of skipjack was not observed.
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