Abstract
?-Glucosidase has an important role in cellulose degradation by cleaving the cellobiose to glucose units. Supplementation ofTrichodermacellulase with exogenous ?-glucosidase is needed to prevent the inhibition effect of cellobiose on exoglucanases and endoglucanases.Production of ?-glucosidase byAspergillus nigerandAspergillus phoenicishas been investigated under different fermentations. Both strains were appropriate for enzyme production at high level under the applied conditions.Cultivation ofA. phoenicison Mandels' medium containing a complex nitrogen source has resulted in a higher ?-glucosidase activity than on Vogel's medium. In an air-lift fermenterA. phoenicisgrew in the shape of beads with 1.41 IU g–1 cell-associated enzyme activity. The fungal pellets can be used as in situ immobilized enzyme preparation.A. nigerproduced extracellular ?-glucosidase at the level of 2.1 IU ml–1 in stirred-tank fermenter with a yield of 210 IU g–1 glucose and productivity of 21.8 IU l–1 h–1.
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