Abstract
Bone marrow-derived lymphocytes (B cells) with or without receptors for a third component of complement (CR) were studied in their responsiveness to the F(ab′) 2 fragment of antiimmunoglobulins (anti-Ig). Spleen cells from C57BL/6J mice were fractionated by the centrifugation over Ficoll-Hypaque density gradient after they were rosetted with erythrocyte-antibody complement complexes. The cells in the interface fraction responded poorly to anti-Ig, while the cells in the pellet fraction responded well. The low responsiveness of CR(−) B cells was confirmed by assaying the responsiveness of cells passed through a Sephadex G-10-complement column. Reduced response of CR(−) B cells could not be explained by the depletion of helper or accessory cells. The relationship between CR, B-cell differentiation and proliferative capacity of B cells is discussed.
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