Abstract
BackgroundThe episodic sub-episode block sums split-integrated weighted average-averaged gene overexpression tropy quotient (esebssiwaagoTQ) is a measure of the 5′ → 3′ reading direction intergene distance tropy that needs to be overcome for horizontal alignment of a gene for maximal transcription; and it is also an arbitrary unit measure of the intracellular pressure needed for maximal gene expression.In this study, B-cell differentiation is studied by esebssiwaagoTQ-based pressuromodulation mapping of B-cell stage marker genes.MethodsLocations of 25 B-cell differentiation stage genes, and locations of downstream and upstream genes were mined at GeneCards and at LNCipedia, pseudogenes included and enhancers excluded. The esebssiwaagoTQs for each gene were determined. A pressuromodulation map was generated by arranging overexpressed B-cell stage marker genes in descending and ascending order by esebssiwaagoTQ in reference to periods of B-cell polarization.ResultsThe gene esebssiwaagoTQs are CD34 0.65 (0.648), PRDM1 0.36 (0.356), PTPRC 0.35 (0.345), MKI67 0.33 (0.329), ENPP1 0.31 (0.308), RAG2 0.31 (0.306), MS4A1 0.30 (0.299), PCNA 0.28 (0.285), ESPL1 0.28 (0.275), CD79B 0.27 (0.271), AICDA 0.27 (0.266), CD40 0.26 (0.257), APOBEC3A/-B 0.22 (0.216), CD38 0.21 (0.212), CD27 0.19 (0.194), APOBEC3C/−D/-F/−G 0.17 (0.173), CD19 0.15 (0.153), RAG1 0.14 (0.139), CD79A 0.14 (0.137), CR2 0.11 (0.109), and APOBEC3H 0.10 (0.102); these are pressuromodulation mapped in reference to B-cell polarization state and differentiation stage.ConclusionsThe esebssiwaagoTQ-based pressuromodulation map of B-cell differentiation simulates the in vivo B-cell maturation process for the classical pathway (T-cell mediated pressuromodulation effect pathway) and applies to the parallel non-classical pathway (T-cell independent antigen-mediated pressuromodulation effect pathway). Henceforth the B-cell pressuromodulation map can be utilized as the template for the study of specific B-cell events including bi-allelic V(D)J gene recombination, IGHM internal consensus recognition sequence, IGHD homologous recombination or initial allelic exclusion, further consensus recognition sequence isotype switchings, and somatic hypermutation, as in Part II.
Highlights
The episodic sub-episode block sums split-integrated weighted average-averaged gene overexpression tropy quotient is a measure of the 5′ → 3′ reading direction intergene distance tropy that needs to be overcome for horizontal alignment of a gene for maximal transcription; and it is an arbitrary unit measure of the intracellular pressure needed for maximal gene expression
In the case of the myeloid bone marrow cells, the various hematopoietic lineage cell types in the sub-cortical marrow caverns are subject to equivalent surrounding tissue cell pressuromodulation by the non-synergistic macropressuromodulation effect, which results in a decrease in effective intracellular pressure due to the presence of extracellular pressure [2, 3]; whereas, their common progenitor stem cell group at the cortical subcortical cavern interface is subject to synergistic cell membrane pressuromodulation in the vascularly pressurized biological system [2, 3], which results in an increase in intracellular pressure by the synergistic macro-pressuromodulation effect (Elastancecell* Pressureintracellular = k)
The esebssiwaagoTQ is a measure of the 5′ → 3′ reading direction intergene distance tropy that needs to be overcome for horizontal alignment of a gene for maximal transcription [3]; it is an arbitrary unit measure of the intracellular pressure needed for maximal gene expression
Summary
The episodic sub-episode block sums split-integrated weighted average-averaged gene overexpression tropy quotient (esebssiwaagoTQ) is a measure of the 5′ → 3′ reading direction intergene distance tropy that needs to be overcome for horizontal alignment of a gene for maximal transcription; and it is an arbitrary unit measure of the intracellular pressure needed for maximal gene expression. The esebssiwaagoTQ is a measure of the 5′ → 3′ reading direction intergene distance tropy that needs to be overcome for horizontal alignment of a gene for maximal transcription [3]; it is an arbitrary unit measure of the intracellular pressure needed for maximal gene expression. The gene esebssiwaagoTQ is accurate to 3-significant digits (Infra, < 0.245; Supra, ≥ 0.245 < 0.745) for Episode 2 category (> 11,864 ≤ 265,005 base), Episode 3 category (≤ 11,864 base) and Episode 6 category (≥ 2,241,933 base) genes, which are the majority of human genes; and it is accurate to 2-significant digits (Infra, < 0.25; Supra, ≥ 0.25 < 0.75) for Episode 4 category (> 265,005 < 607,463 base) and Episode 5 category (≥ 607,463 < 2,241,933 base) genes, which are the minority of them [2, 3]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
