Abstract
Memory T cells (TMEM) play a crucial role in the rejection of allogeneic transplants and represents a major barrier to tolerance of allografts. Some evidence has been provided showing that mice lacking B cells (uMT mice) fail to develop anamnestic T cell immunity after allotransplantation. In the present study, we investigated the effects of in vivo pre-transplant B cell depletion using an anti-CD20 mAb (18-B12) on the development of TMEM responses in OT1 and OT2 mice (anti-OVA TCR transgenic mice) transplanted with an OVA Tg (mAct-OVA) skin graft. B cell depletion had no effect on acute graft rejection and it did not alter the percentages of CD4+ and CD8+ (CD44+CD62L+/-) TMEMs present in the recipients' blood and secondary lymphoid organs after transplantation. However, B cell-depleted mice mounted much higher early effector alloresponses than untreated mice but failed to develop any long-term CD4+ and CD8+ memory T cell response (direct alloresponse measured at d40 post-transplantation). Therefore, anti-CD20-mediated B cell depletion prevents the development of memory T cell direct alloreactivity after allotransplantation.
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