Abstract
Abstract Background In legal medicine, the determination Blood Alcohol Content (BAC) usually faced the problems of insufficient sample size, ethanol degradation and impossible double check. In addition, the integrity of laboratory may also be questioned. To this end, we developed a decentralized solution based on isotope dilution method. Methods Patented deuterated ethanol vacuum tube was used in field as regular type, weight of blood was record to BACer terminal. Arbitrary amount of sample(ca.0.1-1.0g) was pipetted to GC sample vial and sealed tightly, then heated more than 2min at 70°C, 10µL headspace sample was injected into GC-MS. Blinded lab uploaded peak area ratio of ethanol/deuterated ethanol(mz31/33) to BACer to calculate the final BAC. Results Regression between peak area ratios of ethanol/deuterated ethanol showed good linearity in the range of 0.10-5.0 (R2>0.999). Regression equation of different instruments were proved good consistency with slopes ranged from 1.03 to 1.07, and the LOD and LOQ were around 0.01mg/g and 0.05mg/g, 3-level recoveries between 97%-101% with RSD less than 1%. During the 6-month stability test at 4°C and normal temperature, the blood sample test results maintained good stability. The proposed method was applied to 2021 CNAS proficiency testing (2021SF-CNAS002) organized by China Academy of Forensic Sciences with satisfactory results. The comparison with Chinese standard method SF/Z JD0107001-2010 yielded good agreement with Passing&Bablok test (P>0.10). The expanded uncertainty estimated by Monte-carlo method was less than 3%. Conclusions This proposed method showed the possibility of decentralized determination of BAC with lab blinding, the separation of on-site sampling data and laboratory testing also provided the possibility of integrity of results. It also provided a possible solution for the scenarios where the storage condition of blood collection is poor, or the cross check is required. But good chromatographic separation and operation are compulsive due to the possibility of interference in IDMS.
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