Azospirillum brasilense activates peroxidase-mediated cell wall modification to inhibit root cell elongation

Abstract

The molecular mechanism of beneficial bacterium Azospirillum brasilense-mediated root developmental remain elusive. A.brasilense elicited extensively transcriptional changes but inhibited primary root elongation in Arabidopsis. By analyzing root cell type-specific developmental markers, we demonstrated that A.brasilense affected neither overall organization nor cell division of primary root meristem. The cessation of primary root resulted from reduction of cell elongation, which is probably because of bacterially activated peroxidase that will lead to cell wall cross-linking at consuming of H2O2. The activated peroxidase combined with downregulated cell wall loosening enzymes consequently led to cell wall thickness, whereas inhibiting peroxidase restored root growth under A.brasilense inoculation. We further showed that peroxidase activity was probably promoted by cadaverine secreted by A.brasilense. These results suggest that A.brasilense inhibits root elongation by activating peroxidase and inducing cell wall modification in Arabidopsis, in which cadaverine released by A.brasilense is a potential signal compound.