Axonal variability of GABAergic ivy interneurons in the hippocampal CA1 area

Abstract

Event Abstract Back to Event Axonal variability of GABAergic ivy interneurons in the hippocampal CA1 area D. Asede1, M. Capogna1, K. Lamsa2, D. Lapray1*, W. Nissen2, P. Somogyi1 and A. Szabo2 1 University of Oxford, Medical Research Council, Anatomical Neuropharmacology Unit, United Kingdom 2 University of Oxford, Department of Pharmacology, United Kingdom Pyramidal cells in CA1 receive spatially segregated glutamatergic input from the CA3 area in stratum oriens and radiatum on the one hand, and from the entorhinal cortex in stratum lacunosum moleculare on the other. Both local GABAergic ivy and neurogliaform cells express NOS and NPY, have a dense axonal field, and mediate slow synaptic transmission. However, ivy cells associate their axonal arbour with the CA3 input, whereas neurogliaform cells associate their axonal arbour with the entorhinal cortex input. This suggests an input pathway specific modulation of pyramidal cell activity by these cell types. Here we explored the variability in the axonal output of these two related interneuron types by recording cells in acute brain slices in vitro from juvenile rats (P21-28) and labelling them with neurobiotin. Individual ivy cells innervated either mainly stratum oriens, or stratum radiatum, or both equally. Here we show that unlike the axon of previously reported ivy cells, some of these interneurons also innervate stratum lacunosum moleculare to various degrees. Individual neurogliaform cells innervated only stratum lacunosum moleculare, or in addition the molecular layer of the dentate gyrus, or stratum lacunosum moleculare and stratum radiatum. Thus, it appears that there is overlap between the axonal output of ivy and neurogliaform cell populations. Because the dendritic trees of these interneurons may be completely within one of the two major glutamatergic input zones to CA1, their axons innervating the other input zone could potentially provide cross-modulatory influence. It needs to be further explored if the frequency of cells with axons of different laminar distribution varies along the septo-temporal axis of the hippocampus and whether they express different signalling molecules. Conference: EMBO workshop: Gaba Signalling and Brain Networks , Amsterdam, Netherlands, 30 Jun - 2 Jul, 2010. Presentation Type: Poster Presentation Topic: Posters Citation: Asede D, Capogna M, Lamsa K, Lapray D, Nissen W, Somogyi P and Szabo A (2010). Axonal variability of GABAergic ivy interneurons in the hippocampal CA1 area. Conference Abstract: EMBO workshop: Gaba Signalling and Brain Networks . doi: 10.3389/conf.fnins.2010.15.00013 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 24 Jun 2010; Published Online: 24 Jun 2010. * Correspondence: D. Lapray, University of Oxford, Medical Research Council, Anatomical Neuropharmacology Unit, Oxford, United Kingdom, damien.lapray@pharm.ox.ac.uk Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers D. Asede M. Capogna K. Lamsa D. Lapray W. Nissen P. Somogyi A. Szabo Google D. Asede M. Capogna K. Lamsa D. Lapray W. Nissen P. Somogyi A. Szabo Google Scholar D. Asede M. Capogna K. Lamsa D. Lapray W. Nissen P. Somogyi A. Szabo PubMed D. Asede M. Capogna K. Lamsa D. Lapray W. Nissen P. Somogyi A. Szabo Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.