Axonal outgrowth stimulation after alginate/mesenchymal stem cell therapy in injured rat spinal cord

Abstract

Despite strong efforts in the field, spinal cord trauma still belongs among the untreatable neurological conditions at present. Given the complexity of the nervous system, an effective therapy leading to complete recovery has still not been found. One of the potential tools for supporting tissue regeneration may be found in mesenchymal stem cells, which possess anti‑inflammatory and trophic factor‑producing properties. In the context of transplantations, application of degradable biomaterials which could form asupportive environment and scaffold to bridge the lesion area represents another attractive strategy. In the present study, through acombination of these two approaches we applied both alginate hydrogel biomaterial alone or allogenic transplants of MSCs isolated from bone marrow seeded in alginate biomaterial into injured rat spinal cord at threeweeks after spinal cord compression performed at Th8‑9 level. Following three‑week survival, using immunohistochemistry we studied axonal growth (GAP‑43 expression) and both microglia (Iba‑1) and astrocyte (GFAP) reactions at the lesion site and in the segments below and above the lesion. To detect functional improvement, during whole survival period we performed behavioral analyses of locomotor abilities using aclassical open field test (BBB score) and aCatwalk automated gait analyzing device (Noldus). We found that despite the absence of locomotor improvement, application of both alginate and MSCs caused significant increase in the number of GAP‑43 positive axons.