Abstract
Studies of the requirements of Anthonomus grandis Boheman for the indispensable amino acids were extended. Semiquantitative requirements for histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine were established for weevils grown on an amino acid diet that had proved satisfactory previously. Tryrosine neither replaced nor spared phenylalanine when it was substituted for all or part of the phenylalanine. The boll weevil was found to require dietary biotin when it was fed an amino acid diet, but no requirement for vitamin B12 was observed for a single generation. Four generations of boll weevils were reared under aseptic conditions when both larvae and adults were fed a sterile defined diet.
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