Abstract
Salvinia has potential as an experimental organism because of its small size, simplicity, and rapid growth. A limited amount of work has been done on reproduction and growth of Salvinia. Cultural studies employing nonsterile conditions have revealed information on vegetative growth (Rajan, Betteridge & Blackman, 1971), photoperiodic responses (Nakayama, 1952), and competitive growth (Chatworthy & Harper, 1962). The effect of growth regulators (Gaudet & Huang, 1967; Gaudet & Koh, 1968) and growth in a simulated natural environment (Gaudet, 1973) have been studied using aseptic culture. It is the intent of this note to report a simple, reproducible method by which Salvinia can be surfacesterilized and cultured aseptically.
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