Abstract
Cultures of Thalassia testudinum were established and maintained in the absence of other detectable organisms. Axenic cultures were initiated using surface sterilized seeds which were aseptically dissected from surface sterilized fruits. Seedlings were cultured in 75-ml (25-mm × 200-mm) culture tubes containing 30 ml of rooting substrate and 40 ml of chemically defined seawater media. Seedlings and culture media were analyzed for microbial contamination after 42 days of culture utilizing standard marine bacterial/fungal isolating procedures and by light and scanning electron microscopy. Axenic seagrass cultures allow physiological studies such as nutrient assimilation kinetics, rhizosphere and phyllosphere microbial interactions through mono- and poly-axenic seagrass-microbial cultures.
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