Abstract
The use of an avidin—biotin system in the preparation of enzyme sensors is described. The biotin-labelled enzymes can be immobilized on the avidin-modified electrode surface through avidin—biotin complexation. The various techniques for the surface derivatization with biotin and avidin and for the coupling with enzymes are cited. The possibility of constructing a protein architecture on the electrode is also discussed: this is based on the non-covalent interaction of avidin and biotin.
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