Abstract
We have determined the terminal heteropolymeric sequences of AMV RNA by the following procedures: first, RNA sequence determination on the 5′ terminal and the poly(A)-linked 3′ terminal T1 oligonucleotides, and second, analysis by the Maxam and Gilbert (1977) method of AMV strong stop DNA and of DNA complementary to the poly(A)-linked T1 oligonucleotide, synthesized with reverse transcriptase and (pdT) 13 as primer. The structure deduced for the 5′ terminal region is (5′)7mGpppG mCCAUUCUACCUCUCACCACAUUG-GUGUGCACCUGGGUUGAUGGCCGGACCGUCGA-UUCCCUGACGACUACGAGCACCUGCAUGAAGC- AGAAGGCUUCAU … Two distinct 3′ terminal sequences were deduced: GCCAUUCUACCUCU-CAAA … A OH and GCCAUUCUACCUCUCAC-CAAA … A OH. The two termini, differing by a C-C-A sequence, may reflect genetic heterogeneity of the AMV stock or, more probably, may be generated at or after RNA transcription. These results demonstrate a terminal redundancy of the hetero polymeric sequence of 16 and 19 nucleotides, respectively. The terminal redundancy allows for mechanisms which involve transfer of the DNA segment synthesized on the 5′ terminal redundant sequence to the 3′ terminal redundant sequence.
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