Abstract
sBackgroundAvian leukosis virus subgroup J (ALV-J) is an oncogenic retrovirus which causes immunosuppression and neoplasia in meat-type and egg-type chickens. ALV-J infects host cells via specific interaction between the viral Env and the cell surface receptor —chicken sodium hydrogen exchanger type 1 (chNHE1). NHE1 involved in altering the cellular pH and playing a critical role in tumorigenesis. However, little is known about the other relationship between ALV-J and chNHE1.Methods and resultsIn ALV-J infected DF-1 cells, the mRNA level of chNHE1 was up-regulated with time-dependent manner tested by real time PCR, and accordingly, intracellular pH was increased tested by spectrofluorometer. In vivo, the mRNA level of chNHE1 was determined by real time PCR in ALV-J infected experimental chickens and field cases. The result showed that the mRNA level of chNHE1 was up-regulated after virus shedding, especially in continuous viremic shedders (CS group). However, no significant difference was found between non-shedding group (NS group) and control group. In field cases, mRNA level of chNHE1 was positively correlated with increasing ALV-J load in tumor bearing and immune tolerance chickens. Furthermore, immunohistochemistry results showed that the protein expression of chNHE1 was up-regulated in different organs of both experimental chickens and tumor bearing chickens compared with the control.ConclusionTaken together, we conclude that ALV-J induces chNHE1 up-regulation in viremia and neoplasia chickens.
Highlights
Avian leukosis virus subgroup J (ALV-J) is an oncogenic retrovirus which causes immunosuppression and neoplasia in meat-type and egg-type chickens
Transcription of chicken sodium hydrogen exchanger type 1 (chNHE1) and measurement of intracellular pH in Avian leukosis viruses (ALVs)-J infected DF-1 cells To investigate whether ALV-J regulates chNHE1 expression and affects the pHi in vitro, we tested mRNA level of chNHE1 and pHi value in ALV-J infected DF-1 cells
The results indicated that ALV-J up-regulates the expression of chNHE1 with time-dependent manner
Summary
Avian leukosis virus subgroup J (ALV-J) is an oncogenic retrovirus which causes immunosuppression and neoplasia in meat-type and egg-type chickens. ALV-J infects host cells via specific interaction between the viral Env and the cell surface receptor —chicken sodium hydrogen exchanger type 1 (chNHE1). Avian leukosis viruses (ALVs) are a group of avian oncogenic retroviruses which cause immunosuppression and tumors in host birds. Six subgroups (A–E and J) of ALV isolated from chicken are classified according to the virus neutralization, viral interference and the host range [1]. Subgroup J (ALV-J) was reported in 1991 and broke out in meat-type and egg-type chickens during the 2000s [2,3,4,5,6,7].
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