Abstract
SUMMARYA system based on a commercial microcomputer and a commercial image‐capturing TV interface is described which carries out both real space and conventional Fourier transform‐based image averaging procedures. Various real space manipulations are described for handling images with either one‐dimensional or two‐dimensional periodic features. In certain cases, real space averaging procedures have many advantages and are recommended to those not familiar with the mathematics of methods based on Fourier transformation. In other cases Fourier transformation processes are necessary and these also have been implemented on the microcomputer. They can be used to determine periodicities, to produce two‐dimensional average images or as a basis for three‐dimensional reconstruction. Examples showing the principles of application of these methods use appropriate images from electron micrographs of muscle and collagen.
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