Abstract
To assess the natural pregnancy and to determine the morphofunctional aspects of ovaries of rabbits submitted to bilateral oophorectomy and orthotopic allogeneic or autologous intact and sliced ovarian transplantation without a vascular pedicle. Fifty-six female New Zealand White and California rabbits were studied. The ovaries were removed and orthotopically transplanted or replaced without vascular anastomoses: Group 1 (n = 8), only laparotomy and laparorrhaphy were performed; Group 2A (n = 8) intact ovaries were reimplanted on both sides; Group 2B (n = 8) both ovaries were sliced and orthotopically reimplanted; Group 2C (n = 8), an intact ovary was reimplanted on one side and a sliced ovary on the other side; Group 3A (n = 8) intact ovaries were transplanted on both sides, Group 3B (n = 8) both ovaries were sliced and orthotopically transplanted, Group 3C (n = 8), an intact ovary was transplanted on one side and a sliced ovary on the other side. Three months later, the females were paired with males for copulation. Estradiol, progesterone, follicle stimulating hormone and luteinizing hormone levels were assessed. The morphological aspect of the ovaries was studied and the number of pregnancies and litters were also determined. The number of successful pregnancies and the number of litters was compared between the groups by the chi-square test. One-way ANOVA and the Tukey-Kramer tests compared the hormonal dosages. The significance was of p < 0.05. Pregnancies occurred in seven (87.5%) rabbits of Group 1, in 37.5% in Groups 2A and 3A, in 50% of groups 2B, 2C and 3B, and in 62.5% of group 3C. Hormone levels and histology confirmed the vitality of all ovaries. Intact or sliced orthotopic allogeneic and autologous ovarian transplantation without a vascular pedicle is viable in rabbits, and preserves their fertility and hormonal functions.
Highlights
Pregnancies occurred in seven (87.5%) rabbits of Group 1, in 37.5% in Groups 2A and 3A, in 50% of groups 2B, 2C and 3B, and in 62.5% of group 3C
Intact or sliced orthotopic allogeneic and autologous ovarian transplantation without a vascular pedicle is viable in rabbits, and preserves their fertility and hormonal functions. [Rev Assoc Med Bras 2006; 52(6): 447-52]
Viabilidade e função endócrina de auto-implantes ovarianos com drenagem pela veia porta ou cava
Summary
ANDY PETROIANU*, LUIZ RONALDO ALBERTI, LEONARDO DE SOUZA VASCONCELLOS Trabalho realizado no Departamento de Cirurgia, Faculdade de Medicina da Universidade Federal de Minas Gerais, Belo Horizonte, MG. O objetivo do presente trabalho foi verificar a ocorrência de gestação natural em coelhas e avaliar aspectos morfológicos e endócrinos de reimplantes ou transplantes ovarianos homógenos, fixados em posição ortotópica, na forma íntegra e fatiada, sem anastomose vascular. Todos os implantes ovarianos foram feitos sem reconstituição vascular e fixados com apenas um ponto de náilon 5-0, de acordo com os subgrupos seguintes: subgrupo 3A – transplante bilateral dos ovários íntegros; subgrupo 3B – os ovários foram cortados longitudinalmente em três fatias de 2 mm de espessura com bisturi e, em seguida, foram transplantados; subgrupo 3C – transplantou-se aleatoriamente de um lado o ovário íntegro e do outro o fatiado. As coelhas do Grupo 3 receberam diariamente, durante nove meses, 10 mg/kg de ciclosporina dissolvidos em 10 ml de leite, através de cateter orogástrico com calibre de 12 Fr. Os animais foram semanalmente pesados para ajuste das doses de ciclosporina. Retiraram-se o útero, os ovários e as tubas uterinas de todas as coelhas, que foram processados para estudo histológico
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