AVALIAÇÃO DA PRESENÇA DO POLIMORFISMO SstI DA APOLIPOPROTEÍNA C-III E SUA ASSOCIAÇÃO COM OS NÍVEIS DE TRIGLICERÍDEOS EM UMA AMOSTRA DE DIABÉTICOS NO SUL DO BRASIL

Abstract

Introduction: Type 2 diabetes mellitus (DM2) is the most common metabolic disease. Objective: Toinvestigate the presence of the SstI polymorphism of the C-III apolipoprotein and verify the associationwith the lipid profile in a diabetic sample from Southern Brazil. Materials and methods: Sixty-fivepatients with DM2 were subjected to anthropometric measurements, lipid profile and polymorphismanalyses. The SstI polymorphism of APO C-III was made using the polymerase chain reaction methodfollowed by enzymatic digestion (PCR-RFLP). Fragments that showed a size of 269 base pairs (bp) and159 bp were classified as G allele, and fragments that showed a size of 428 bp were classified as C allele.We used the Student t test to compare the means of the lipid profile between men and women while thepolymorphism analysis was performed using the chi-square test (?2) and the Fisher exact test Results:The mean age was 64.67±8.74 and BMI was 1.09±4.86 kg/m2. Of the 65 individuals, 55% did not useantilipemic medication. The rate of total cholesterol, triglycerides, HDL and LDL levels were withinnormal limits. Regarding polymorphism, 80% of the subjects had normal genotype (CC), 3.1% changedgenotype (GG), and 16.9% altered genotype (CG). There was no significant association between CCgenotype and triglyceride levels below 200 mg/dL. Conclusion: In the evaluated population, the majorityof the individuals presented a normal genotype to the SstI polymorphism with no association with thetriglycerides rate.