Abstract
The aim of this study was to evaluate the rate of water absorption and cytochemical changes in primed and aged seeds of sorghum during germination under absence or presence of 100 mM NaCl. The initial batch of seeds of sorghum cv. IPA-1011, was divided into two batches of different levels of vigor, by the accelerated aging of half seeds, with half of each batch also referred to the priming of polyethylene glycol 6000 at -0.86 MPa for 48 hours. Four replicates of 50 seeds from each batch were placed on two sheets of filter paper, blotting paper saturated with distilled water and placed inside gerboxes (11 x 11 x 3.5 cm) transparent lids, which were kept in a germination chamber under continuous darkness and 25 oC for 72 hours. The cytochemical analysis characterization was measured in the soaking times of 0; 24; 48 and 72 hours. The sorghum seeds cells showed irregular shapes, ranging from elliptical to rounded, and the staining with the toluidine blue also revealed the presence of nuclei, especially in primed seeds, indicating that some cells showed high cellular activity. The presence of protein was detected mainly in protein bodies on cytoplasm of cotyledon cells, while the starch was identified in the form of granules at endosperm. The accelerated aging treatments and seed priming, associated to salt stress resulted in little morphological and cytochemical visible changes during the experimental period analyzed.
Highlights
The aim of this study was to evaluate the rate of water absorption and cytochemical changes in primed and aged seeds of sorghum during germination under absence or presence of 100 mM NaCl
A coloração com azul de toluidina (AT) foi utilizada para a análise morfológica das células cotiledonares das sementes de sorgo, em cortes transversais, nos quais foram verificados padrões semelhante de coloração em todas as condições analisadas (FIG. 1)
As análises citoquímicas com o corante xylidine Ponceau (XP) detectaram a presença de proteínas no citoplasma das células cotiledonares das sementes de sorgo, armazenadas em vacúolos de reserva, sendo observadas poucas alterações nessas células nos diferentes tratamentos analisados (FIG. 2)
Summary
The aim of this study was to evaluate the rate of water absorption and cytochemical changes in primed and aged seeds of sorghum during germination under absence or presence of 100 mM NaCl. A coloração com azul de toluidina (AT) foi utilizada para a análise morfológica das células cotiledonares das sementes de sorgo, em cortes transversais, nos quais foram verificados padrões semelhante de coloração em todas as condições analisadas
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
